Regulatory

Part:BBa_K5178014

Designed by: Virginia iGEM 2024   Group: iGEM24_Virginia   (2024-10-02)


P43_lac o_MF002

This sequence contains the constitutive P43 promoter, the lac operator (lac o) sequence, and the MF002 RBS. Designed for B. subtilis, the lac repressor protein (LacI) can bind the lac o sequence within the promoter region, preventing transcription. Thus, the transcription of a gene of interest is modulated by the presence of LacI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 75
    Illegal NheI site found at 79
    Illegal NheI site found at 88
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None